ABSTRACT
Two new 2-carboxymethyl-3-hexyl-maleic anhydride derivatives, arthrianhydride A (1) and B (2), along with three known compounds 3-5, were isolated from the fermentation broth of a grasshopper-associated fungus Arthrinium sp. NF2410. The structures of new compounds 1 and 2 were determined based on the analysis of the HR-ESI-MS and NMR spectroscopic data. Furthermore, compounds 1 and 2 were evaluated on inhibitory activity against the enzyme SHP2 and both of them showed moderate inhibitory activity against SHP2.
Subject(s)
Animals , Anhydrides/pharmacology , Biological Products/pharmacology , Enzyme Inhibitors/pharmacology , Fungi/chemistry , Grasshoppers/microbiology , Molecular Structure , Protein Tyrosine Phosphatase, Non-Receptor Type 11/antagonists & inhibitors , Secondary MetabolismABSTRACT
This work addresses the production of prodigiosin from ram horn peptone (RHP) using MO-1, a local isolate in submerged culture. First, a novel gram-negative and rod-shaped bacterial strain, MO-1, was isolated from the body of the grasshopper (Poecilemon tauricola Ramme 1951), which was collected from pesticide-contaminated fields. Sequence analysis of 16S rDNA classified the microbe as Serratia marcescens. The substrate utilization potential (BIOLOG) and fatty acid methyl ester profile (FAME) of S. marcescens were also determined. The effect of RHP on the production of prodigiosin by S. marcescens MO-1 was investigated, and the results showed that RHP supplementation promoted the growth of MO-1 and increased the production of prodigiosin. A concentration of 0.4% (w/v) RHP resulted in the greatest yield of prodigiosin (277.74 mg/L) after 48 h when mannitol was used as the sole source of carbon. The pigment yield was also influenced by the types of carbon sources and peptones. As a result, RHP was demonstrated to be a suitable substrate for prodigiosin production. These results revealed that prodigiosin could be produced efficiently by S. marcescens using RHP.
Subject(s)
Animals , Culture Media/chemistry , Peptones/metabolism , Prodigiosin/metabolism , Serratia marcescens/growth & development , Serratia marcescens/metabolism , Bacterial Typing Techniques , Cluster Analysis , Cytosol/chemistry , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Fatty Acids/analysis , Grasshoppers/microbiology , Molecular Sequence Data , Phylogeny , Pigments, Biological/metabolism , /genetics , Sequence Analysis, DNA , Serratia marcescens/classification , Serratia marcescens/isolation & purificationABSTRACT
A otimizaçäo da produçäo de conídios de Metarhizium flavoviride (CG 423) foi investigada variando a temperatura, teor inicial de água e substratos visando ao desenvolvimento deste patógeno como um bioinseticida contra gafanhotos, uma séria praga da agricultura brasileira. A produçäo de conídios foi altamente afetada pela temperatura, tendo sido otimizada quando o fungo foi cultivado a 27ºC. O teor de água no substrato também influenciou a produçäo de conídios. A quantidade ótima de água ficou entre 30 e 120p/cento (v/w) dependendo do substrato. Os substratos testados foram arroz parboilizado, arroz comum, quirela de arroz e palha + farelo de arroz e arroz parboilizado, arroz comum, quirela de arroz e palha + farelo de arroz. Os melhores substratos para produçäo de conídios de M. flavoviride foram palha de arroz + farelo de arroz e arroz parboilizado